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plasmid pmod b2303  (Addgene inc)


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    Structured Review

    Addgene inc plasmid pmod b2303
    Plasmid Pmod B2303, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmod+b2303/pMOD_B2303+(Plasmid+%2391068)/pmc12856575-105-0-2
    Average 93 stars, based on 7 article reviews
    plasmid pmod b2303 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Modification:

    Article Title: Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene
    Article Snippet: .. The pMOD_A0101, modified pMOD_B2303, and empty vector pMOD_C0000 (Addgene #91081) were assembled into a non-binary vector, pTRANS_100 (Addgene #91198) and binary vector pTRANS_210 (Addgene plasmid # 91108) by Golden Gate protocol using the Aar I enzyme. ..

    Article Title: Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene.
    Article Snippet: .. The pMOD_A0101, modified pMOD_B2303, and empty vector pMOD_C0000 (Addgene #91081) were assembled into a non-binary vector, pTRANS_100 (Addgene #91198) and binary vector pTRANS_210 (Addgene plasmid # 91108) by Golden Gate protocol using the AarI enzyme. ..

    Article Title: Multiplex CRISPR-Cas9 editing of chlorophyll biosynthesis genes in chickpea via protoplast and Agrobacterium-mediated transformation.
    Article Snippet: Chickpea (Cicer arietinum L.) is considered one of the most important legumes due to its high nutrient content and ability to fix nitrogen symbiotically (Jukanti et al. 2012).. Chickpeas complement the bioavailability of nutrients when consumed with cereals due to their high protein content (18–22%), which is twice that of wheat and three times that of rice (Yegrem 2021).. Due to the astounding attributes of chickpeas, it is imperative to enhance their production to meet nutritional requirements and promote the overall wellbeing of the global population (Talip et al. 2018).

    Article Title: Optimization of Protoplast Isolation and Transformation for a Pilot Study of Genome Editing in Peanut by Targeting the Allergen Gene Ara h 2
    Article Snippet: The synthesized pUC57-PTG was digested with Pst I and Xho I and cloned into the Pst I and Xho I-digested pMOD_B2303 vector (Addgene #91068) using T4 Ligase (NEB) following the manufacturer’s recommendations ( ). .. Modified pMOD_A0101, modified pMOD_B2303, and empty vector pMOD_C0000 (Addgene #91081) were assembled into a non-binary vector, pTRANS_100 (Addgene #91198) by simple Golden Gate protocol using the Aar I enzyme [ ] ( ). .. All steps were performed according to the manufacturer’s instructions for in vitro digestion of DNA with Cas9 nuclease (NEB), with a few modifications.

    Plasmid Preparation:

    Article Title: Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene
    Article Snippet: .. The pMOD_A0101, modified pMOD_B2303, and empty vector pMOD_C0000 (Addgene #91081) were assembled into a non-binary vector, pTRANS_100 (Addgene #91198) and binary vector pTRANS_210 (Addgene plasmid # 91108) by Golden Gate protocol using the Aar I enzyme. ..

    Article Title: Promoter‐pervasive transcription causes RNA polymerase II pausing to boost DOG1 expression in response to salt
    Article Snippet: pMOD_A0402 , Čermák et al ( ) , Addgene Plasmid #91009. .. pMOD_B2303 , Čermák et al ( ) , Addgene Plasmid #91068. .. pMOD_C3001 , Čermák et al ( ) , Addgene Plasmid #91094.

    Article Title: Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene.
    Article Snippet: .. The pMOD_A0101, modified pMOD_B2303, and empty vector pMOD_C0000 (Addgene #91081) were assembled into a non-binary vector, pTRANS_100 (Addgene #91198) and binary vector pTRANS_210 (Addgene plasmid # 91108) by Golden Gate protocol using the AarI enzyme. ..

    Clone Assay:

    Article Title: Dual activities of an X-family DNA polymerase regulate CRISPR-induced insertional mutagenesis across species
    Article Snippet: .. In brief, the gRNAs, listed in Supplementary Data , were first cloned into pMOD_B2301 (for Arabidopsis ; Addgene #91067) or pMOD_B2303 (for Setaria ; Addgene #91068) to create gRNA expression plasmids. .. The Setaria protoplast transfection constructs were generated by using the Golden Gate assembly method with pMOD_A1110 (the Cas9 expressing plasmid, Addgene #91031), the gRNA plasmids, pMOD_C3001 (the GFP reporter plasmid; Addgene #91094), and pTRANS100 (the destination plasmid; Addgene #91198).

    Article Title: Dual Activities of an X-family DNA Polymerase Regulate CRISPR-Induced Insertional Mutagenesis Across Species
    Article Snippet: 355 Vector construction 356 Plasmids for Arabidopsis and Setaria transformation and CRISPR-Cas9 mutagenesis 357 experiments were created using the method described previously 34. .. The gRNAs, listed in 358 Supplementary Table 1, were first cloned into pMOD_B2301 (for Arabidopsis; Addgene 359 #91067) or pMOD_B2303 (for Setaria; Addgene #91068) to create gRNA expression plasmids. .. 360 The Setaria protoplast transfection constructs were generated by using the Golden Gate assembly 361 method with pMOD_A1110 (the Cas9 expressing plasmid, Addgene #91031), the gRNA 362 plasmids, pMOD_C3001 (the GFP reporter plasmid; Addgene #91094), and pTRANS100 (the 363 destination plasmid; Addgene #91198).

    Expressing:

    Article Title: Dual activities of an X-family DNA polymerase regulate CRISPR-induced insertional mutagenesis across species
    Article Snippet: .. In brief, the gRNAs, listed in Supplementary Data , were first cloned into pMOD_B2301 (for Arabidopsis ; Addgene #91067) or pMOD_B2303 (for Setaria ; Addgene #91068) to create gRNA expression plasmids. .. The Setaria protoplast transfection constructs were generated by using the Golden Gate assembly method with pMOD_A1110 (the Cas9 expressing plasmid, Addgene #91031), the gRNA plasmids, pMOD_C3001 (the GFP reporter plasmid; Addgene #91094), and pTRANS100 (the destination plasmid; Addgene #91198).

    Article Title: Dual Activities of an X-family DNA Polymerase Regulate CRISPR-Induced Insertional Mutagenesis Across Species
    Article Snippet: 355 Vector construction 356 Plasmids for Arabidopsis and Setaria transformation and CRISPR-Cas9 mutagenesis 357 experiments were created using the method described previously 34. .. The gRNAs, listed in 358 Supplementary Table 1, were first cloned into pMOD_B2301 (for Arabidopsis; Addgene 359 #91067) or pMOD_B2303 (for Setaria; Addgene #91068) to create gRNA expression plasmids. .. 360 The Setaria protoplast transfection constructs were generated by using the Golden Gate assembly 361 method with pMOD_A1110 (the Cas9 expressing plasmid, Addgene #91031), the gRNA 362 plasmids, pMOD_C3001 (the GFP reporter plasmid; Addgene #91094), and pTRANS100 (the 363 destination plasmid; Addgene #91198).



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